BioA

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  • Description: lysine-8-amino-7-oxononanoate aminotransferase

Gene name bioA
Synonyms
Essential no
Product lysine-8-amino-7-oxononanoate aminotransferase
Function biosynthesis of biotin
Gene expression levels in SubtiExpress: bioA
Metabolic function and regulation of this protein in SubtiPathways:
bioA
MW, pI 49 kDa, 5.32
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours bioF, bioW
Sequences Protein DNA DNA_with_flanks
Genetic context
BioA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BioA expression.png

























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5350 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2260 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2738 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications

Scott W Van Arsdell, John B Perkins, R Rogers Yocum, Linda Luan, C Linda Howitt, Nilu Prasad Chatterjee, Janice G Pero
Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction.
Biotechnol Bioeng: 2005, 91(1);75-83
[PubMed:15880481] [WorldCat.org] [DOI] (P p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)