GatB

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  • Description: production of glutamyl-tRNA(Gln)

Gene name gatB
Synonyms yerN
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase (sununit B)
Function translation
Gene expression levels in SubtiExpress: gatB
Interactions involving this protein in SubtInteract: GatB
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 53 kDa, 4.898
Gene length, protein length 1428 bp, 476 aa
Immediate neighbours gatA, yerO
Sequences Protein DNA DNA_with_flanks
Genetic context
GatB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GatB expression.png















Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06690

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: GatB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

Akiyoshi Nakamura, Min Yao, Sarin Chimnaronk, Naoki Sakai, Isao Tanaka
Ammonia channel couples glutaminase with transamidase reactions in GatCAB.
Science: 2006, 312(5782);1954-8
[PubMed:16809541] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)