SacC

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  • Description: levanase

Gene name sacC
Synonyms
Essential no
Product levanase
Function degradation of levan to fructose
Gene expression levels in SubtiExpress: sacC
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 75 kDa, 6.789
Gene length, protein length 2031 bp, 677 aa
Immediate neighbours yraA, levG
Sequences Protein DNA DNA_with_flanks
Genetic context
SacC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SacC expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, LevR regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU27030

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed

Maria Elena Ortiz-Soto, Enrique Rudiño-Piñera, Maria Elena Rodriguez-Alegria, Agustin Lopez Munguia
Evaluation of cross-linked aggregates from purified Bacillus subtilis levansucrase mutants for transfructosylation reactions.
BMC Biotechnol: 2009, 9;68
[PubMed:19635162] [WorldCat.org] [DOI] (I e)

I Martin-Verstraete, J Stülke, A Klier, G Rapoport
Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon.
J Bacteriol: 1995, 177(23);6919-27
[PubMed:7592486] [WorldCat.org] [DOI] (P p)

M Débarbouillé, I Martin-Verstraete, F Kunst, G Rapoport
The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria.
Proc Natl Acad Sci U S A: 1991, 88(20);9092-6
[PubMed:1924373] [WorldCat.org] [DOI] (P p)

M Débarbouillé, I Martin-Verstraete, A Klier, G Rapoport
The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators.
Proc Natl Acad Sci U S A: 1991, 88(6);2212-6
[PubMed:1900939] [WorldCat.org] [DOI] (P p)

I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
J Mol Biol: 1990, 214(3);657-71
[PubMed:2117666] [WorldCat.org] [DOI] (P p)

I Martin, M Débarbouillé, E Ferrari, A Klier, G Rapoport
Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase.
Mol Gen Genet: 1987, 208(1-2);177-84
[PubMed:3112519] [WorldCat.org] [DOI] (P p)