RecA

• Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
  
  

Gene name	recA
Synonyms	recE
Essential	no
Product	multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
Function	DNA repair/ recombination
Interactions involving this protein in SubtInteract: RecA
MW, pI	37 kDa, 4.883
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	cinA, pbpX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon, LexA regulon

The gene

Basic information

• Locus tag: BSU16940

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

RecA filaments are dismantled from DNA by PcrA PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • RecA stimulates ssDNA phosphorylase activity of PnpA PubMed

• Protein family: recA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:
  • RecO and DprA provide RecA access to ssDNA during chromosomal transformation PubMed

• Interactions:
  • RecA-DprA PubMed
  • RecA-RecU PubMed
  • RecA-ComFA PubMed
  • SsbB-RecA PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • Nucleoid (Mid-cell) PubMed
  • localizes to one cell pole PubMed

Database entries

• Structure: 1U94 (RecA from E. coli, 62% identity, 86% similarity)

• UniProt: P16971

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed
  • induced by conditions that trigger development of genetic competence (ComK) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: IRN444 (cat), available in the Stülke lab

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Reviews

Silvia Ayora, Begoña Carrasco, Paula P Cárdenas, Carolina E César, Cristina Cañas, Tribhuwan Yadav, Chiara Marchisone, Juan C Alonso
Double-strand break repair in bacteria: a view from Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(6);1055-81
[PubMed:21517913] [WorldCat.org] [DOI] (I p)

Justin Courcelle, Philip C Hanawalt
RecA-dependent recovery of arrested DNA replication forks.
Annu Rev Genet: 2003, 37;611-46
[PubMed:14616075] [WorldCat.org] [DOI] (P p)

Michael M Cox
The bacterial RecA protein as a motor protein.
Annu Rev Microbiol: 2003, 57;551-77
[PubMed:14527291] [WorldCat.org] [DOI] (P p)

Shelley L Lusetti, Michael M Cox
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
Annu Rev Biochem: 2002, 71;71-100
[PubMed:12045091] [WorldCat.org] [DOI] (P p)

M M Cox
Recombinational DNA repair in bacteria and the RecA protein.
Prog Nucleic Acid Res Mol Biol: 1999, 63;311-66
[PubMed:10506835] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed