DnaK

• Description: class I heat-shock protein (molecular chaperone)
  
  

Gene name	dnaK
Synonyms
Essential	no
Product	class I heat-shock protein (molecular chaperone)
Function	protein quality control
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	65 kDa, 4.571
Gene length, protein length	1833 bp, 611 aa
Immediate neighbours	surC, grpE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: heat shock protein 70 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DnaK-DnaJ

• Localization: cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) PubMed

Database entries

• Structure:

• UniProt: P17820

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage