Difference between revisions of "DprA"
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− | * '''Description:''' conveys incoming ssDNA to [[RecA]]; facilitates the displacement of both SSBs ([[SsbB]] and [[SsbA]]), increases [[RecA]] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing <br/><br/> | + | * '''Description:''' conveys incoming ssDNA to [[RecA]]; facilitates the displacement of both SSBs ([[SsbB]] and [[SsbA]]), increases [[RecA]] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex <br/><br/> |
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Revision as of 15:49, 21 August 2014
- Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex
Gene name | dprA |
Synonyms | smf |
Essential | no |
Product | recombination mediator protein |
Function | DNA repair/ recombination |
Gene expression levels in SubtiExpress: dprA | |
Interactions involving this protein in SubtInteract: DprA | |
MW, pI | 32 kDa, 9.755 |
Gene length, protein length | 891 bp, 297 aa |
Immediate neighbours | sucD, topA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA repair/ recombination, genetic competence
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16110
Phenotypes of a mutant
- strongly impaired genetic recombination PubMed
Database entries
- BsubCyc: BSU16110
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: smf family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU16110
- Structure:
- UniProt: P39813
- KEGG entry: [3]
Additional information
Expression and regulation
- Operon: dprA (according to DBTBS)
- Sigma factor:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 57 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
References
Tribhuwan Yadav, Begoña Carrasco, James Hejna, Yuki Suzuki, Kunio Takeyasu, Juan C Alonso
Bacillus subtilis DprA recruits RecA onto single-stranded DNA and mediates annealing of complementary strands coated by SsbB and SsbA.
J Biol Chem: 2013, 288(31);22437-50
[PubMed:23779106]
[WorldCat.org]
[DOI]
(I p)
Nicolas Mirouze, Mathieu A Bergé, Anne-Lise Soulet, Isabelle Mortier-Barrière, Yves Quentin, Gwennaele Fichant, Chantal Granadel, Marie-Françoise Noirot-Gros, Philippe Noirot, Patrice Polard, Bernard Martin, Jean-Pierre Claverys
Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.
Proc Natl Acad Sci U S A: 2013, 110(11);E1035-44
[PubMed:23440217]
[WorldCat.org]
[DOI]
(I p)
Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918]
[WorldCat.org]
[DOI]
(I p)
Serkalem Tadesse, Peter L Graumann
DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells.
BMC Microbiol: 2007, 7;105
[PubMed:18045469]
[WorldCat.org]
[DOI]
(I e)
Isabelle Mortier-Barrière, Marion Velten, Pauline Dupaigne, Nicolas Mirouze, Olivier Piétrement, Stephen McGovern, Gwennaele Fichant, Bernard Martin, Philippe Noirot, Eric Le Cam, Patrice Polard, Jean-Pierre Claverys
A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA.
Cell: 2007, 130(5);824-36
[PubMed:17803906]
[WorldCat.org]
[DOI]
(P p)
Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974]
[WorldCat.org]
[DOI]
(P p)
Janet C Lindow, Masayoshi Kuwano, Shigeki Moriya, Alan D Grossman
Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein.
Mol Microbiol: 2002, 46(4);997-1009
[PubMed:12421306]
[WorldCat.org]
[DOI]
(P p)
Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146]
[WorldCat.org]
[DOI]
(P p)