SsbB

• Description: single-strand DNA-binding protein
  
  

Gene name	ssbB
Synonyms	ywpH
Essential	no
Product	single-strand DNA-binding protein
Function	required for efficient genetic transformation
Gene expression levels in SubtiExpress: ssbB
Interactions involving this protein in SubtInteract: SsbB
MW, pI	12 kDa, 6.876
Gene length, protein length	339 bp, 113 aa
Immediate neighbours	glcR, ywpG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, genetic competence, DNA repair/ recombination, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU36310

Database entries

• BsubCyc: BSU36310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds and protects internalized foreign ssDNA PubMed

• Protein family:

• Paralogous protein(s): SsbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-55 PubMed
  • phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed

• Cofactors:

• Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed

• Interactions:
  • forms tetramers PubMed
  • SsbB-ComFA PubMed
  • SsbB-CoiA PubMed
  • SsbB-DprA PubMed
  • SsbB-RecA PubMed

• Localization:
  • localizes to cell poles in competent cells PubMed
  • localizes to the DNA entry pole during transformation PubMed

Database entries

• BsubCyc: BSU36310

• Structure: 3VDY 3VDY (in complex with ssDNA) PubMed

• UniProt: C0SPB6

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ssbB PubMed
  • ssbB-glcR PubMed (this is unlikely according to PubMed)

• Expression browser: ssbB PubMed

• Sigma factor:

• Regulation:
  • expressed at the onset of stationary phase PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 144 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 617 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 473 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1758 PubMed

Biological materials

• Mutant:

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)