glcR

168

transcriptional repressor ([wiki|DeoR family]) of the [gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]-[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC] operon

locus: BSU_36300

Molecular weight: 28.67 kDa

pI: 5.69

Protein length: 258 aa

Gene length: 777 bp

function: unknown

product: transcriptional repressor ([wiki|DeoR family])

essential: no

synonyms: glcR, ywpI

List of homologs in different organisms, belongs to COG1349 (Galperin et al., 2021)

Gene

Coordinates: 3,739,206  3,739,982

The protein

Protein family

• [wiki|DeoR family] of transcription factors

[wiki|Domains]

• [wiki|HTH deoR-type domain] (aa 3-58) (according to UniProt)

Structure

• [AF|P94591]

Additional information

• Mutant forms of GlcR act as "super-repressors" of'' [gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]'' expression [Pubmed|11491085]

Expression and Regulation

Operons

• genes: [gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]-[gene|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC]

  description: [Pubmed|30782637,22383849]

  regulation

  • induced by phosphosugar stress [Pubmed|30782637]

  regulatory mechanism

  • [protein|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]: repression, [pubmed|30782637], in [regulon|protein:6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|30782637], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A530 (ywpI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/530 NBRP B. subtilis, Japan]
• BKE36300 ([gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCCCTCATTCCTTTTCT,  downstream forward: _UP4_GATGAAGGAAAGGACTGACA
• BKK36300 ([gene|6F5831A480B72D17B7B3F0BF7AF147603B65CA49|glcR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36300 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCCCTCATTCCTTTTCT,  downstream forward: _UP4_GATGAAGGAAAGGACTGACA

References

• 11491085,14762004,11948146,22383849,30782637,38282124