NarG

• Description: nitrate reductase (alpha subunit)
  
  

Gene name	narG
Synonyms
Essential	no
Product	nitrate reductase (alpha subunit)
Function	nitrate respiration, nitrogen assimilation
Gene expression levels in SubtiExpress: narG
Interactions involving this protein in SubtInteract: NarG
MW, pI	138 kDa, 6.061
Gene length, protein length	3684 bp, 1228 aa
Immediate neighbours	narH, arfM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon

The gene

Basic information

• Locus tag: BSU37280

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

• Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • Fe-S cluster PubMed
  • MoCo PubMed

• Effectors of protein activity:

• Interactions:
  • part of a respiratory supercomplex PubMed
  • NarG-NarH PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: P42175

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: narG-narH-narJ-narI PubMed

• Expression browser: narG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • Fnr: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Pedro M F Sousa, Marco A M Videira, Filipe A S Santos, Brian L Hood, Thomas P Conrads, Ana M P Melo
The bc:caa3 supercomplexes from the Gram positive bacterium Bacillus subtilis respiratory chain: a megacomplex organization?
Arch Biochem Biophys: 2013, 537(1);153-60
[PubMed:23880299] [WorldCat.org] [DOI] (I p)

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)