HrcA

• Description: transcriptional repressor of class I heat-shock genes
  
  

Gene name	hrcA
Synonyms	yqxE, yqeS
Essential	no
Product	transcriptional repressor
Function	regulation of chaperone gene expression
Gene expression levels in SubtiExpress: hrcA
Interactions involving this protein in SubtInteract: HrcA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	38 kDa, 6.079
Gene length, protein length	1029 bp, 343 aa
Immediate neighbours	grpE, hemN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, heat shock proteins

This gene is a member of the following regulons

HrcA regulon

The HrcA regulon:

• hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV, groES-groEL

The gene

Basic information

• Locus tag: BSU25490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repressor of the * hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV and groES-groEL operons (at low/ normal temperatures)

• Protein family: hrcA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): GroEL acts as co-repressor

• Effectors of protein activity:

• Interactions: GroEL-HrcA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P25499

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Expression browser: hrcA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

Thomas Wiegert, Wolfgang Schumann
Analysis of a DNA-binding motif of the Bacillus subtilis HrcA repressor protein.
FEMS Microbiol Lett: 2003, 223(1);101-6
[PubMed:12799007] [WorldCat.org] [DOI] (P p)

Silke Reischl, Thomas Wiegert, Wolfgang Schumann
Isolation and analysis of mutant alleles of the Bacillus subtilis HrcA repressor with reduced dependency on GroE function.
J Biol Chem: 2002, 277(36);32659-67
[PubMed:12082092] [WorldCat.org] [DOI] (P p)

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

A Mogk, G Homuth, C Scholz, L Kim, F X Schmid, W Schumann
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.
EMBO J: 1997, 16(15);4579-90
[PubMed:9303302] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

A Schulz, W Schumann
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.
J Bacteriol: 1996, 178(4);1088-93
[PubMed:8576042] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)

A Schulz, B Tzschaschel, W Schumann
Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis.
Mol Microbiol: 1995, 15(3);421-9
[PubMed:7540247] [WorldCat.org] [DOI] (P p)

U Zuber, W Schumann
CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis.
J Bacteriol: 1994, 176(5);1359-63
[PubMed:8113175] [WorldCat.org] [DOI] (P p)

M Wetzstein, U Völker, J Dedio, S Löbau, U Zuber, M Schiesswohl, C Herget, M Hecker, W Schumann
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
J Bacteriol: 1992, 174(10);3300-10
[PubMed:1339421] [WorldCat.org] [DOI] (P p)