Difference between revisions of "MmgA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mmgB]]'', ''[[yqiK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mmgB]]'', ''[[yqiK]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24170 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24170 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mmgA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mmgA_context.gif]]

Revision as of 10:40, 14 May 2013

  • Description: degradative acetoacetyl-CoA thiolase

Gene name mmgA
Synonyms yqiL
Essential no
Product degradative acetoacetyl-CoA thiolase
Function mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: mmgA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 41 kDa, 5.904
Gene length, protein length 1179 bp, 393 aa
Immediate neighbours mmgB, yqiK
Sequences Protein DNA DNA_with_flanks
Genetic context
MmgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MmgA expression.png




























Categories containing this gene/protein

sporulation proteins, utilization of amino acids

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

  • Locus tag: BSU24170

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 acetyl-CoA = CoA + acetoacetyl-CoA PubMed
  • Protein family: thiolase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3SS6 (the protein of B. anthracis, 44% identity, 73% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Jason J Reddick, Jayme K Williams
The mmgA gene from Bacillus subtilis encodes a degradative acetoacetyl-CoA thiolase.
Biotechnol Lett: 2008, 30(6);1045-50
[PubMed:18246303] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)