fadA

168

acetyl-CoA C-acyltransferase

Locus: BSU_32830

Molecular weight: 40.96 kDa

Isoelectric point: 5.18

Protein length: 391 aa

Gene length: 1176 bp

Function: fatty acid degradation

Product: acetyl-CoA C-acyltransferase

Essential: no

E.C.: 2.3.1.16

Synonyms: fadA, yusK

Genomic Context

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List of homologs in different organisms, belongs to COG0183 (Galperin et al., 2021)

Gene

Coordinates: 3,368,839  3,370,014

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + acyl-CoA --> 3-oxoacyl-CoA + CoA (according to UniProt)

Protein family

• thiolase-like superfamily (according to UniProt)

Structure

• 3SS6 (PDB) (the protein of B. anthracis, 41% identity, 73% similarity)
• O32177 (AlphaFold)

Paralogous protein(s)

• MmgA, YhfS

Expression and Regulation

Operons

• Genes: fadN-fadA-fadE

  Description: PubMed

  Regulation

  • ''fadN'': induced by long-chain fatty acids (FadR) PubMed

  Regulatory mechanism

  • SdpR: activation, PubMed, in sdpR regulon
  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadM-fadN-fadA-fadE

  Description: PubMed

  Regulation

  • ''fadM'': induced by long-chain fatty acids (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • SdpR: activation, PubMed, in sdpR regulon

Biological materials

Mutant

• MGNA-A603 (yusK::erm), available at the NBRP B. subtilis, Japan
• BKE32830 (fadA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT,  downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA
• BKK32830 (fadA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT,  downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA

References

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