mmgB

168

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

Locus: BSU_24160

Molecular weight: 26.92 kDa

Isoelectric point: 6.6

Protein length: 287 aa

Gene length: 864 bp

Function: mother cell metabolism

Product: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

Essential: no

E.C.: 1.1.1.157

Synonyms: mmgB, yqiM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1250 (Galperin et al., 2021)

Gene

Coordinates: 2,511,973 → 2,512,836

The protein

Catalyzed reaction/ biological activity

• (3S)-hydroxybutanoyl-CoA + NADP⁺ --> acetoacetyl-CoA + H⁺ + NADPH (according to UniProt)

Protein family

• 3-hydroxyacyl-CoA dehydrogenase family (with FadN, according to UniProt)

Structure

• 4R1N (PDB) (from Clostridium butyricum, 56% identity) PubMed
• P45856 (AlphaFold)

Expression and Regulation

Operons

• Genes: mmgA-mmgB-mmgC-mmgD-mmgE-mmgF

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE24160 (ΔmmgB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAAATCCCCTTTTTCA,  downstream forward: _UP4_TGAAGTCTATTCCATCCGGG
• BKK24160 (ΔmmgB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAAATCCCCTTTTTCA,  downstream forward: _UP4_TGAAGTCTATTCCATCCGGG

References

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