Difference between revisions of "BirA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU22440 birA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22440 birA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.769   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.769   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[panB]]'', ''[[cca]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[panB]]'', ''[[cca]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14160&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22440 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:birA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:birA_context.gif]]

Revision as of 13:08, 13 May 2013

  • Description: trigger enzyme: transcriptional repressor of the biotin operon / biotin-protein ligase

Gene name birA
Synonyms
Essential yes PubMed
Product trigger enzyme: transcriptional repressor/ biotin-protein ligase
Function regulation of biotin synthesis, addition of biotin to proteins
Gene expression levels in SubtiExpress: birA
MW, pI 36 kDa, 6.769
Gene length, protein length 975 bp, 325 aa
Immediate neighbours panB, cca
Sequences Protein DNA Advanced_DNA
Genetic context
BirA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BirA expression.png
























Categories containing this gene/protein

biosynthesis of lipids, biosynthesis of cofactors, transcription factors and their control, trigger enzyme, essential genes

This gene is a member of the following regulons

The BirA regulon:

The gene

Basic information

  • Locus tag: BSU22440

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • transcriptional repressor of the bioW-bioA-bioF-bioD-bioB-bioI-ytbQ operon PubMed and
    • ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] (according to Swiss-Prot)
  • Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)

S Bower, J Perkins, R R Yocum, P Serror, A Sorokin, P Rahaim, C L Howitt, N Prasad, S D Ehrlich, J Pero
Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.
J Bacteriol: 1995, 177(9);2572-5
[PubMed:7730294] [WorldCat.org] [DOI] (P p)

Operon and expression

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)