Difference between revisions of "BioA"

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Revision as of 11:25, 20 April 2012

  • Description: lysine-8-amino-7-oxononanoate aminotransferase

Gene name bioA
Synonyms
Essential no
Product lysine-8-amino-7-oxononanoate aminotransferase
Function biosynthesis of biotin
Metabolic function and regulation of this protein in SubtiPathways:
Biotin
MW, pI 49 kDa, 5.32
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours bioF, bioW
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BioA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BioA expression.png

























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications

Scott W Van Arsdell, John B Perkins, R Rogers Yocum, Linda Luan, C Linda Howitt, Nilu Prasad Chatterjee, Janice G Pero
Removing a bottleneck in the Bacillus subtilis biotin pathway: bioA utilizes lysine rather than S-adenosylmethionine as the amino donor in the KAPA-to-DAPA reaction.
Biotechnol Bioeng: 2005, 91(1);75-83
[PubMed:15880481] [WorldCat.org] [DOI] (P p)

J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842] [WorldCat.org] [DOI] (P p)

S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940] [WorldCat.org] [DOI] (P p)