CotX

• Description: spore coat protein (insoluble fraction), necessary for the assembly of the spore crust, the outermost layer of the spore coat
  
  

Gene name	cotX
Synonyms
Essential	no
Product	spore coat protein (insoluble fraction)
Function	spore coat assembly
MW, pI	18 kDa, 4.228
Gene length, protein length	516 bp, 172 aa
Immediate neighbours	cotY, cotW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU11760

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • spore wall (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q08313

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotV-cotW-cotX PubMed

cotX PubMed

• Sigma factor: before cotV: SigK PubMed

before cotX: SigK PubMed

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism: upstream of cotX: SpoIIID: repression PubMed, upstream of both promoters: GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: