cotV

168

spore crust protein (insoluble fraction), formation of the spore crust

Locus: BSU_11780

Molecular weight: 14.09 kDa

Isoelectric point: 4.01

Protein length: 128 aa

Gene length: 387 bp

Function: resistance of the spore

Product: spore crust protein (insoluble fraction)

Essential: no

Synonyms: cotV

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5886 (Galperin et al., 2021)

Gene

Coordinates: 1,251,631  1,252,017

The protein

Structure

• Q08309 (AlphaFold)

Localization

• spore crust PubMed

Expression and Regulation

Operons

• Genes: cotV-cotW-cotX

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon
  • SpoIIID: activation, PubMed, in spoIIID regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon
  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE11780 (cotV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACCTTCACTCCTTTCT,  downstream forward: _UP4_TAACCCTTTCTTTTCACAAA
• BKK11780 (cotV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACCTTCACTCCTTTCT,  downstream forward: _UP4_TAACCCTTTCTTTTCACAAA

References

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