SpoIVCB

• Description: sporulation-specific sigma factor (SigK) (N-terminal half), other part: spoIIIC

Gene name	spoIVCB
Synonyms	cisB, sigK
Essential	no
Product	RNA polymerase sporulation-specific sigma factor (sigma-K) (N-terminal half)
Function	late mother cell-specific gene expression
MW, pI	17 kDa, 8.208
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	nucB, spoIVCA
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU25760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by SigK

gerE, lipC, surC, yabG, yndL, yitB-yitA-yisZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIVFB-proSigK PubMed
  • SigK-(RpoB-RpoC)

• Localization:

Database entries

• Structure:

• UniProt: P12254

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigK (composed of spoIVCB-spoIIIC) PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed
  • SpoIIID: transcription activation PubMed

• Additional information:
  • SigK is activated by processing of membrane-bound pro-SigK by the SpoIVFB metalloprotease PubMed
  • spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage
• Charles Moran, Emory University, NC, USA homepage