spoIVCA

168

site-specific DNA recombinase required for creating the sigK gene (excision of the Skin element)

Locus: BSU_25770

Molecular weight: 57.31 kDa

Isoelectric point: 9.5

Protein length: 500 aa

Gene length: 1503 bp

Function: excision of the Skin element, creation of the sigK gene

Product: site-specific DNA recombinase

Essential: no

Synonyms: spoIVCA, cisA

Genomic Context

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List of homologs in different organisms, belongs to COG1961 (Galperin et al., 2021)

Gene

Coordinates: 2,653,371  2,654,873

The protein

Protein family

• N-terminal part: site-specific recombinase resolvase family (according to UniProt)

Domains

• Resolvase/invertase-type recombinase catalytic domain (aa 1-144) (according to UniProt)

Structure

• P17867 (AlphaFold)

Expression and Regulation

Operons

• Genes: spoIVCA

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: activation, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE25770 (spoIVCA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTGAATCTCCTTCCTA,  downstream forward: _UP4_TTTCAATCAATCGGTGCAGA
• BKK25770 (spoIVCA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTGAATCTCCTTCCTA,  downstream forward: _UP4_TTTCAATCAATCGGTGCAGA

References

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