Difference between revisions of "GatA"

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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || glutamyl-tRNA(Gln) amidotransferase sununit A
+
|style="background:#ABCDEF;" align="center"| '''Product''' || glutamyl-tRNA(Gln) amidotransferase subunit A
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[GatA]]-[[GatB]]-[[GatC]]
+
* '''Interactions:'''  
 +
** ([[GatA]]-[[GatB]]-[[GatC]])-[[GltX]]-[[trnS-Gln]] (the "transamidosome") {{PubMed|20882017}}, see a [http://www.nature.com/nature/journal/v467/n7315/fig_tab/nature09411_F3.html model of the interaction] {{PubMed|20882017}}
  
 
* '''Localization:'''
 
* '''Localization:'''
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=References=
 
=References=
  
<pubmed>9342321,, </pubmed>
+
<pubmed>9342321, 20882017, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:44, 14 October 2010

  • Description: production of glutamyl-tRNA(Gln)

Gene name gatA
Synonyms yerM, yedB
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase subunit A
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 52 kDa, 5.24
Gene length, protein length 1455 bp, 485 aa
Immediate neighbours gatC, gatB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GatA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU06680

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: amidase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)