Difference between revisions of "AcoL"

From SubtiWiki
Jump to: navigation, search
Line 72: Line 72:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' ([[AcoA]]-[[AcoB]])-[[AcoC]]-[[AcoL]]
  
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
 
* '''Localization:''' cytoplasm (according to Swiss-Prot)
Line 100: Line 100:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[CcpA]]: transcription repression {{PubMed|10666464}}
 
** [[CcpA]]: transcription repression {{PubMed|10666464}}
** [[AcoR]]: transcription activation (interaction with [[SigL]]-containing RNA polymerase) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
+
** [[AcoR]]: transcription activation (interaction with [[SigL]]-containing [[RNA polymerase]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
 
** [[Fnr]]: indirect positive regulation via [[NarG]]-[[NarH]]-[[NarJ]]-[[NarI]] {{PubMed|16428414}}
 
** [[Fnr]]: indirect positive regulation via [[NarG]]-[[NarH]]-[[NarJ]]-[[NarI]] {{PubMed|16428414}}
  

Revision as of 18:00, 21 August 2010

  • Description: acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)

Gene name acoL
Synonyms yfjH
Essential no
Product acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Function acetoin utilization
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 48 kDa, 5.273
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours acoC, acoR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AcoL context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU08090

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)
  • Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References