LpdV

• Description: 2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
  
  

Gene name	lpdV
Synonyms	yqiV, bkd
Essential	no
Product	2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
Function	utilization of branched-chain keto acids
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val
MW, pI	48 kDa, 4.893
Gene length, protein length	1371 bp, 457 aa
Immediate neighbours	bkdAA, buk
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

• Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2YQU (from Thermus thermophilus (hb8 mutant), 42% identity, 55% similarity)

• UniProt: P54533

• KEGG entry: [3]

• E.C. number: 1.8.1.4

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induced in the presence of isoleucine or valine (BkdR) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • BkdR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: