Spo0A

• Description: phosphorelay regulator, initiation of sporulation
  
  

Gene name	spo0A
Synonyms	spo0C, spo0G, spoIIL, sof-1
Essential	no
Product	phosphorelay response regulator
Function	initiation of sporulation
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Ammonium/ glutamate, Central C-metabolism, Sugar catabolism, Stress, tRNA charging
MW, pI	29 kDa, 5.989
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	yqiG, spoIVB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24220

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Genes controlled by Spo0A

• Activation by Spo0A: spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG

• Repression by Spo0A: abrB, kinA, kinC, spo0A, metS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed

• Cofactor(s):

• Effectors of protein activity: the phosphorylation state affects DNA binding activity

• Interactions: KinC-Spo0A PubMed, Spo0A-Spo0E, Spo0A-SigA, Spo0A-Spo0B

• Localization:

Database entries

• Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)

• UniProt: P06534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0A PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732] [WorldCat.org] [DOI] (I p)

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)