Difference between revisions of "GrpE"
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| − | * '''Description:''' heat-shock protein (activation of DnaK) <br/><br/> | + | * '''Description:''' heat-shock protein (activation of [[DnaK]]) <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Product''' || heat-shock protein | |style="background:#ABCDEF;" align="center"| '''Product''' || heat-shock protein | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || control of DnaK activity | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[DnaK]] activity |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]''' | ||
| Line 92: | Line 92: | ||
* '''Operon:''' ''[[hrcA]]-[[grpE]]-[[dnaK]]-[[dnaJ]]-[[yqeT]]-[[yqeU]]-[[yqeV]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9023197 PubMed] | * '''Operon:''' ''[[hrcA]]-[[grpE]]-[[dnaK]]-[[dnaJ]]-[[yqeT]]-[[yqeU]]-[[yqeV]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9023197 PubMed] | ||
| − | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1339421}} |
| − | * '''Regulation:''' induced by heat shock ([[HrcA]]) | + | * '''Regulation:''' |
| + | ** induced by heat shock ([[HrcA]]) {{PubMed|1339421}} | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| + | ** [[HrcA]]: transcription repression {{PubMed|1339421}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 18:24, 22 November 2009
- Description: heat-shock protein (activation of DnaK)
| Gene name | grpE |
| Synonyms | |
| Essential | no |
| Product | heat-shock protein |
| Function | control of DnaK activity |
| Metabolic function and regulation of this protein in SubtiPathways: Stress | |
| MW, pI | 21 kDa, 4.264 |
| Gene length, protein length | 561 bp, 187 aa |
| Immediate neighbours | dnaK, hrcA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU25480
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: grpE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: Cytoplasm (Homogeneous) PubMed
Database entries
- Structure:
- UniProt: P15874
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Wolfgang Schumann, Bayreuth University, Germany Homepage
Your additional remarks
References
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760]
[WorldCat.org]
[DOI]
(P p)
G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197]
[WorldCat.org]
[DOI]
(P p)
M Wetzstein, U Völker, J Dedio, S Löbau, U Zuber, M Schiesswohl, C Herget, M Hecker, W Schumann
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
J Bacteriol: 1992, 174(10);3300-10
[PubMed:1339421]
[WorldCat.org]
[DOI]
(P p)
