Difference between revisions of "Sandbox"

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#REDIRECT [[bacD]]
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* '''Description:''' 2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase) <br/><br/>
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''lpdV''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiV, bkd ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || 2-oxoisovalerate dehydrogenase <br/>(E3 subunit, dihydrolipoamide dehydrogenase)
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 4.893 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1371 bp, 457 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bkdAA]]'', ''[[buk]]''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14337&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:lpdV_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|}
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__TOC__
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<br/><br/><br/>
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=The gene=
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 +
=== Basic information ===
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 +
* '''Coordinates:'''
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 +
===Phenotypes of a mutant ===
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=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11725]
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=== Additional information===
 +
 
 +
 
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=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:''' Protein N(6)-(dihydrolipoyl)lysine + NAD<sup>+</sup> = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)
 +
 
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* '''Protein family:''' class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''Interactions:'''
 +
 
 +
* '''Localization:'''
 +
 
 +
=== Database entries ===
 +
 
 +
* '''Structure:'''
 +
 
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P54533 P54533]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24060 BSU24060]
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* '''E.C. number:''' [http://www.expasy.org/enzyme/1.8.1.4 1.8.1.4]
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=== Additional information===
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=Expression and regulation=
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* '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]''
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* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
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* '''Regulation:'''
 +
 
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* '''Regulatory mechanism:'''
 +
 
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* '''Additional information:'''
 +
 
 +
=Biological materials =
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* '''Mutant:'''
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* '''Expression vector:'''
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
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* '''two-hybrid system:'''
 +
 
 +
* '''Antibody:'''
 +
 
 +
=Labs working on this gene/protein=
 +
 
 +
=Your additional remarks=
 +
 
 +
=References=
 +
 
 +
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:27, 25 May 2009

  • Description: 2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)

Gene name lpdV
Synonyms yqiV, bkd
Essential no
Product 2-oxoisovalerate dehydrogenase
(E3 subunit, dihydrolipoamide dehydrogenase)
Function utilization of branched-chain keto acids
MW, pI 48 kDa, 4.893
Gene length, protein length 1371 bp, 457 aa
Immediate neighbours bkdAA, buk
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LpdV context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)
  • Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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