Difference between revisions of "FlgM"

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* '''Description:''' anti-[[SigD]], regulation of flagellin, motility, and chemotaxis <br/><br/>
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* '''Description:''' anti-[[SigD]], regulation of flagellin, [[motility and chemotaxis]] <br/><br/>
  
 
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* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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** secreted and extracellularly degraded by the proteases [[Epr]] and [[WprA]] {{PubMed|25313396}}
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** secretion of FlgM requires the [[FliF]]/[[FliG]] basal body proteins, the flagellar type III export apparatus components [[FliO]], [[FliP]], [[FliQ]], [[FliR]], [[FlhA]], and [[FlhB]], and the substrate specificity switch regulator [[FliK]] {{PubMed|25313396}}
  
 
=== Database entries ===
 
=== Database entries ===
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<pubmed>25251856 </pubmed>
 
<pubmed>25251856 </pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed>8955328,19898538 8412657 8045879 , 20233303 10207036 8655488 23352839 21736639</pubmed>
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<pubmed>8955328,19898538 8412657 8045879 , 20233303 10207036 8655488 23352839 21736639 25313396 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:33, 19 October 2014

Gene name flgM
Synonyms
Essential no
Product anti-SigD
Function control of SigD activity
Gene expression levels in SubtiExpress: flgM
Interactions involving this protein in SubtInteract: FlgM
MW, pI 9 kDa, 9.918
Gene length, protein length 264 bp, 88 aa
Immediate neighbours flgN, yvyF
Sequences Protein DNA DNA_with_flanks
Genetic context
FlgM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FlgM expression.png















Categories containing this gene/protein

sigma factors and their control, motility and chemotaxis

This gene is a member of the following regulons

ComK regulon, DegU regulon, ScoC regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU35430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: flgM family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Sampriti Mukherjee, Daniel B Kearns
The structure and regulation of flagella in Bacillus subtilis.
Annu Rev Genet: 2014, 48;319-40
[PubMed:25251856] [WorldCat.org] [DOI] (I p)

Original Publications

Rebecca A Calvo, Daniel B Kearns
FlgM is secreted by the flagellar export apparatus in Bacillus subtilis.
J Bacteriol: 2015, 197(1);81-91
[PubMed:25313396] [WorldCat.org] [DOI] (I p)

Wai Kit Ma, Rachel Hendrix, Claire Stewart, Eric V Campbell, Mitchell Lavarias, Kolyn Morris, Shauna Nichol, Matthew J Gage
FlgM proteins from different bacteria exhibit different structural characteristics.
Biochim Biophys Acta: 2013, 1834(4);808-16
[PubMed:23352839] [WorldCat.org] [DOI] (P p)

Yi-Huang Hsueh, Loralyn M Cozy, Lok-To Sham, Rebecca A Calvo, Alina D Gutu, Malcolm E Winkler, Daniel B Kearns
DegU-phosphate activates expression of the anti-sigma factor FlgM in Bacillus subtilis.
Mol Microbiol: 2011, 81(4);1092-108
[PubMed:21736639] [WorldCat.org] [DOI] (I p)

Loralyn M Cozy, Daniel B Kearns
Gene position in a long operon governs motility development in Bacillus subtilis.
Mol Microbiol: 2010, 76(2);273-85
[PubMed:20233303] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
A dual mode of regulation of flgM by ScoC in Bacillus subtilis.
Can J Microbiol: 2009, 55(8);983-9
[PubMed:19898538] [WorldCat.org] [DOI] (I p)

M G Bertero, B Gonzales, C Tarricone, F Ceciliani, A Galizzi
Overproduction and characterization of the Bacillus subtilis anti-sigma factor FlgM.
J Biol Chem: 1999, 274(17);12103-7
[PubMed:10207036] [WorldCat.org] [DOI] (P p)

K Fredrick, J D Helmann
FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.
J Bacteriol: 1996, 178(23);7010-3
[PubMed:8955328] [WorldCat.org] [DOI] (P p)

T Caramori, D Barilla, C Nessi, L Sacchi, A Galizzi
Role of FlgM in sigma D-dependent gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(11);3113-8
[PubMed:8655488] [WorldCat.org] [DOI] (P p)

D B Mirel, P Lauer, M J Chamberlin
Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis.
J Bacteriol: 1994, 176(15);4492-500
[PubMed:8045879] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, D Dubnau
comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.
Mol Microbiol: 1993, 9(1);119-31
[PubMed:8412657] [WorldCat.org] [DOI] (P p)