scoC

168

transcriptional repressor of genes expressed in the transition phase

Locus: BSU_09990

Molecular weight: 23.57 kDa

Isoelectric point: 5.19

Protein length: 203 aa

Gene length: 612 bp

Function: transition state regulator

Product: transcriptional repressor (MarR family)

Essential: no

Synonyms: scoC, hpr, catA

Genomic Context

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List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)

Gene

Coordinates: 1,073,106 → 1,073,717

Additional information

• the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots PubMed

The protein

Domains

• HTH marR-type domain (aa 13-157) (according to UniProt)

Structure

• 2FXA (PDB)
• P11065 (AlphaFold)

Modification

• phosphorylated on Arg-3 PubMed

Effectors of protein activity

• activity is inhibited in an unknown way by PrkA (possibly phosphorylation) PubMed
• DNA binding is affected by DnmA-mediated DNA methylation PubMed

Expression and Regulation

Operons

• Genes: scoC

  Description: PubMed

  Regulation

  • expressed upon transition into the stationary phase (AbrB) PubMed

  Regulatory mechanism

  • AbrB: activation, PubMed, in abrB regulon
  • SenS: activation, in senS regulon
  • SalA: repression, in salA regulon
  • CodY: repression, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• 1A918 ( scoC::erm  trpC2 leuC7), PubMed, available at the Bacillus Genetic Stock Center
• BKE09990 (ΔscoC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGTCACCTGCTTCTC,  downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
• BKK09990 (ΔscoC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGTCACCTGCTTCTC,  downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab

References

Reviews

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Original Publications

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