Difference between revisions of "SsbA"

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* '''Description:''' single-strand DNA-binding protein, part of the [[replisome]], promoter of [[RecA]] DNA repair center assembly <br/><br/>
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* '''Description:''' single-strand DNA-binding protein, part of the [[replisome]], promoter of [[RecA]] DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex <br/><br/>
  
 
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Revision as of 15:49, 21 August 2014

  • Description: single-strand DNA-binding protein, part of the replisome, promoter of RecA DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex

Gene name ssbA
Synonyms ssb
Essential yes PubMed
Product single-strand DNA-binding protein
Function DNA replication, DNA repair/ recombination
Gene expression levels in SubtiExpress: ssbA
Interactions involving this protein in SubtInteract: SsbA
MW, pI 18 kDa, 4.822
Gene length, protein length 516 bp, 172 aa
Immediate neighbours rpsR, rpsF
Sequences Protein DNA DNA_with_flanks
Genetic context
Ssb context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SsbA expression.png















Categories containing this gene/protein

DNA replication, DNA repair/ recombination, essential genes, phosphoproteins

This gene is a member of the following regulons

ComK regulon, stringent response

The gene

Basic information

  • Locus tag: BSU40900

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): SsbB

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed

Database entries

  • Structure: 3VDY 3VDY (SsbB in complex with ssDNA, 63% identity, 87% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
    • ComK: transcription activation PubMed
  • Additional information:
    • the mRNA contains a structured motif in the 5' UTR upstream of the rpsF ORF that is bound by RpsF-RpsR heterodimers and may be implicated in autogenous regulation PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 279 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2199 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 263 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 147 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3701 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications