Difference between revisions of "Bcd"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[BkdR regulon]]}}, | {{SubtiWiki regulon|[[BkdR regulon]]}}, | ||
| − | |||
{{SubtiWiki regulon|[[SigL regulon]]}} | {{SubtiWiki regulon|[[SigL regulon]]}} | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bcd_2502659_2503753_-1 bcd] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bcd_2502659_2503753_-1 bcd] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed] | + | * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
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<pubmed>7705101</pubmed> | <pubmed>7705101</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>12427936,15241682,10094682,12823818, </pubmed> | + | <pubmed>12427936,15241682,10094682,12823818, 24843172</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:24, 21 May 2014
- Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
| Gene name | bcd |
| Synonyms | bkd, yqiT |
| Essential | no |
| Product | valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase |
| Function | utilization of branched-chain keto acids |
| Gene expression levels in SubtiExpress: bcd | |
| Metabolic function and regulation of this protein in SubtiPathways: bcd | |
| MW, pI | 39 kDa, 4.942 |
| Gene length, protein length | 1092 bp, 364 aa |
| Immediate neighbours | buk, ptb |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24080
Phenotypes of a mutant
Database entries
- BsubCyc: BSU24080
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)
- Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU24080
- UniProt: P54531
- KEGG entry: [3]
- E.C. number: 1.4.1.9
Additional information
Expression and regulation
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3408 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2690 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 11556 PubMed
Biological materials
- Mutant:
- a bcd mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
N M Brunhuber, J S Blanchard
The biochemistry and enzymology of amino acid dehydrogenases.
Crit Rev Biochem Mol Biol: 1994, 29(6);415-67
[PubMed:7705101]
[WorldCat.org]
[DOI]
(P p)
Original publications
