AcoC

• Description: acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
  
  

Gene name	acoC
Synonyms	yfjI
Essential	no
Product	acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)
Function	acetoin utilization
Gene expression levels in SubtiExpress: acoC
Interactions involving this protein in SubtInteract: AcoC
Metabolic function and regulation of this protein in SubtiPathways: acoC
MW, pI	42 kDa, 6.524
Gene length, protein length	1194 bp, 398 aa
Immediate neighbours	acoB, acoL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, sporulation proteins

This gene is a member of the following regulons

AcoR regulon, CcpA regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU08080

Database entries

• BsubCyc: BSU08080

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine (according to Swiss-Prot)

• Protein family: lipoyl-binding domain (according to Swiss-Prot)

• Paralogous protein(s): BkdB, OdhB, PdhC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • lipoic acid

• Effectors of protein activity:

• Interactions:
  • (AcoA-AcoB)-AcoC-AcoL

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• BsubCyc: BSU08080

• Structure:

• UniProt: O31550

• KEGG entry: [3]

• E.C. number: 2.3.1.12

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Expression browser: acoC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by acetoin (AcoR) PubMed
  • induced under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed
  • Fnr: indirect positive regulation via NarG-NarH-NarJ-NarI PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 114 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 325 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 848 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage