Difference between revisions of "SsbA"
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| − | * '''Description:''' single-strand DNA-binding protein, part of the [[replisome]] <br/><br/> | + | * '''Description:''' single-strand DNA-binding protein, part of the [[replisome]], promoter of [[RecA]] DNA repair center assembly <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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** SsbA inhibits ssDNA phosphorylase activity of [[PnpA]] {{PubMed|21859751}} | ** SsbA inhibits ssDNA phosphorylase activity of [[PnpA]] {{PubMed|21859751}} | ||
** SsbA co-assembles onto [[SsbB]]-coated ssDNA {{PubMed|22373918}} | ** SsbA co-assembles onto [[SsbB]]-coated ssDNA {{PubMed|22373918}} | ||
| + | ** important for [[RecA]] DNA repair center assembly by recruiting [[RecO]] and [[RecR]] to the [[replisome]] {{PubMed|24891441}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 172: | Line 173: | ||
<pubmed> 22976189 22933559 </pubmed> | <pubmed> 22976189 22933559 </pubmed> | ||
== Original publications == | == Original publications == | ||
| − | <pubmed>17853894,19730681 11948146,16479537,11948165 16549871 20122408 14762004 20581116 22054219 22373918 22517742 21859751, 21170359, 21958350,23268446 23536821 23779106 22383849 24310371 24443534</pubmed> | + | <pubmed>17853894,19730681 11948146,16479537,11948165 16549871 20122408 14762004 20581116 22054219 22373918 22517742 21859751, 21170359, 21958350,23268446 23536821 23779106 22383849 24310371 24443534 24891441</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:24, 4 June 2014
- Description: single-strand DNA-binding protein, part of the replisome, promoter of RecA DNA repair center assembly
| Gene name | ssbA |
| Synonyms | ssb |
| Essential | yes PubMed |
| Product | single-strand DNA-binding protein |
| Function | DNA replication, DNA repair/ recombination |
| Gene expression levels in SubtiExpress: ssbA | |
| Interactions involving this protein in SubtInteract: SsbA | |
| MW, pI | 18 kDa, 4.822 |
| Gene length, protein length | 516 bp, 172 aa |
| Immediate neighbours | rpsR, rpsF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, DNA repair/ recombination, essential genes, phosphoproteins
This gene is a member of the following regulons
ComK regulon, stringent response
The gene
Basic information
- Locus tag: BSU40900
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU40900
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): SsbB
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold PubMed
- Interactions:
- forms tetramers PubMed
- part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- SsbA-SbcC PubMed
- SsbA-RecG PubMed
- SsbA-YrvE PubMed
- SsbA-YrvN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YqiB PubMed
- SsbA-Ung PubMed
- SsbA-YpbB PubMed
- SsbA-RecD2 PubMed
- SsbA-DnaN PubMed
- SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
- SsbA-YocI according to PubMed
- SsbA-RecQ according to PubMed
Database entries
- BsubCyc: BSU40900
- UniProt: P37455
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
- the mRNA contains a structured motif in the 5' UTR upstream of the rpsF ORF that is bound by RpsF-RpsR heterodimers and may be implicated in autogenous regulation PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 279 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 2199 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 263 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 147 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3701 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Dusica Vujaklija, Boris Macek
Detecting posttranslational modifications of bacterial SSB proteins.
Methods Mol Biol: 2012, 922;205-18
[PubMed:22976189]
[WorldCat.org]
[DOI]
(I p)
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Original publications
