GatB

• Description: production of glutamyl-tRNA(Gln)
  
  

Gene name	gatB
Synonyms	yerN
Essential	yes PubMed
Product	glutamyl-tRNA(Gln) amidotransferase (sununit B)
Function	translation
Gene expression levels in SubtiExpress: gatB
Interactions involving this protein in SubtInteract: GatB
Metabolic function and regulation of this protein in SubtiPathways: gatB
MW, pI	53 kDa, 4.898
Gene length, protein length	1428 bp, 476 aa
Immediate neighbours	gatA, yerO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU06690

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU06690

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)

• Protein family: GatB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GatA-GatB-GatC
  • (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed

• Localization:

Database entries

• BsubCyc: BSU06690

• Structure: 2DF4 (GatA-GatB-GatC complex from Staphylococcus aureus) PubMed

• UniProt: O30509

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: gatB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4822 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 6856 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

Akiyoshi Nakamura, Min Yao, Sarin Chimnaronk, Naoki Sakai, Isao Tanaka
Ammonia channel couples glutaminase with transamidase reactions in GatCAB.
Science: 2006, 312(5782);1954-8
[PubMed:16809541] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)