AbbA

• Description: inhibitor of AbrB activity
  
  

Gene name	abbA
Synonyms	ykzF
Essential	no
Product	anti-repressor
Function	inhibition of AbrB
Gene expression levels in SubtiExpress: abbA
Interactions involving this protein in SubtInteract: AbbA
MW, pI	7 kDa, 4.642
Gene length, protein length	195 bp, 65 aa
Immediate neighbours	ykuK, ykuL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control

This gene is a member of the following regulons

CcpA regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU14120

Database entries

• BsubCyc: BSU14120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibition of AbrB activity PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • AbbA-AbrB, AbbA binds the N-terminal DNA-binding domain of AbrB PubMed

• Localization:

Database entries

• BsubCyc: BSU14120

• Structure: 2LZF PubMed

• UniProt: O31697

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ykuJ-ykuK-abbA-ykuL-ccpC PubMed

• Expression browser: abbA PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (4.3-fold) (CcpA) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Spo0A: transcription activation by Spo0A~P PubMed

• Additional information:

Biological materials

• Mutant: GP214 (deletion of ykuJ-ykuK-abbA-ykuL, replaced by aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP708 (in pAC5), available in Stülke lab, pGP712 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Ashley T Tucker, Benjamin G Bobay, Allison V Banse, Andrew L Olson, Erik J Soderblom, M Arthur Moseley, Richele J Thompson, Kristen M Varney, Richard Losick, John Cavanagh
A DNA mimic: the structure and mechanism of action for the anti-repressor protein AbbA.
J Mol Biol: 2014, 426(9);1911-24
[PubMed:24534728] [WorldCat.org] [DOI] (I p)

Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Irene Reif, Fabian M Commichau, Christian Detsch, Ingrid Wacker, Holger Ludwig, Jörg Stülke
Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system.
Arch Microbiol: 2006, 185(2);136-46
[PubMed:16395550] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)