ykuK

168

unknown

Locus: BSU_14110

Molecular weight: 19.34 kDa

Isoelectric point: 6.42

Protein length: 172 aa

Gene length: 519 bp

Function: unknown

Product: unknown, distantly related to RNase H

Essential: no

Synonyms: ykuK

Genomic Context

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List of homologs in different organisms, belongs to COG1978 (Galperin et al., 2021)

Gene

Coordinates: 1,484,466  1,484,984

The protein

Domains

• DUF458

Structure

• 2E3U (PDB) (from Pyrococcus horikoshii, 24% identity) PubMed
• O34776 (AlphaFold)

Expression and Regulation

Operons

• Genes: ykuJ-ykuK-abbA-darB-ccpC

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, by Spo0A~P PubMed, in spo0A regulon
  • CcpA: repression, PubMed, in ccpA regulon

Biological materials

Mutant

• MGNA-A772 (ykuK::erm), available at the NBRP B. subtilis, Japan
• GP214 (deletion of ''ykuJ-ykuK-abbA-darB, replaced by aphA3''), available in Jörg Stülke's lab
• BKE14110 (ykuK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACGACACTCCTTTTCA,  downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA
• BKK14110 (ykuK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACGACACTCCTTTTCA,  downstream forward: _UP4_TAATTTGGCTGAAAAAGCCA

LacZ fusion

• pGP709 (in pAC5), available in Jörg Stülke's lab, pGP713 (in pAC6), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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