ykuJ

168

unknown

Locus: BSU_14100

Molecular weight: 9.11 kDa

Isoelectric point: 4.34

Protein length: 79 aa

Gene length: 240 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ykuJ

Genomic Context

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List of homologs in different organisms, belongs to COG4703 (Galperin et al., 2021)

Gene

Coordinates: 1,484,117  1,484,356

The protein

Structure

• 2FFG (PDB)
• O34588 (AlphaFold)

Expression and Regulation

Operons

• Genes: ykuJ-ykuK-abbA-darB-ccpC

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, by Spo0A~P PubMed, in spo0A regulon
  • CcpA: repression, PubMed, in ccpA regulon

Biological materials

Mutant

• MGNA-A771 (ykuJ::erm), available at the NBRP B. subtilis, Japan
• GP214 (deletion of ''ykuJ-ykuK-abbA-darB, replaced by aphA3''), available in Jörg Stülke's lab
• BKE14100 (ykuJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT,  downstream forward: _UP4_TAAGATTCCTGAAATAGGGG
• BKK14100 (ykuJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT,  downstream forward: _UP4_TAAGATTCCTGAAATAGGGG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

LacZ fusion

• pGP710 (in pAC5), available in Jörg Stülke's lab, pGP714 (in pAC6), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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