Difference between revisions of "SsbB"
| Line 43: | Line 43: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[DNA replication]]}}, | {{SubtiWiki category|[[DNA replication]]}}, | ||
| + | {{SubtiWiki category|[[genetic competence]]}}, | ||
{{SubtiWiki category|[[DNA repair/ recombination]]}}, | {{SubtiWiki category|[[DNA repair/ recombination]]}}, | ||
{{SubtiWiki category|[[phosphoproteins]]}} | {{SubtiWiki category|[[phosphoproteins]]}} | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| Line 87: | Line 88: | ||
** phosphorylated on Tyr by [[PtkA]], dephosphorylated by [[PtpZ]] {{PubMed|16549871}} | ** phosphorylated on Tyr by [[PtkA]], dephosphorylated by [[PtpZ]] {{PubMed|16549871}} | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' phosphorylation strongly increases DNA-binding activity {{PubMed|16549871}} | * '''Effectors of protein activity:''' phosphorylation strongly increases DNA-binding activity {{PubMed|16549871}} | ||
| Line 104: | Line 105: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId= 3VDY 3VDY] (in complex with ssDNA) {{PubMed|22373918}} | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId= 3VDY 3VDY] (in complex with ssDNA) {{PubMed|22373918}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SPB6 C0SPB6] | * '''UniProt:''' [http://www.uniprot.org/uniprot/C0SPB6 C0SPB6] | ||
Revision as of 11:24, 16 March 2014
- Description: single-strand DNA-binding protein
| Gene name | ssbB |
| Synonyms | ywpH |
| Essential | no |
| Product | single-strand DNA-binding protein |
| Function | required for efficient genetic transformation |
| Gene expression levels in SubtiExpress: ssbB | |
| Interactions involving this protein in SubtInteract: SsbB | |
| MW, pI | 12 kDa, 6.876 |
| Gene length, protein length | 339 bp, 113 aa |
| Immediate neighbours | glcR, ywpG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, genetic competence, DNA repair/ recombination, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36310
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- binds and protects internalized foreign ssDNA PubMed
- Protein family:
- Paralogous protein(s): SsbA
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed
Database entries
- UniProt: C0SPB6
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- expressed at the onset of stationary phase PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Original publications
