MecA

• Description: buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK and ComS to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A∼P by direct interaction
  
  

Gene name	mecA
Synonyms
Essential	no
Product	adaptor protein
Function	control of ComK degradation, regulation of competence
Gene expression levels in SubtiExpress: mecA
Interactions involving this protein in SubtInteract: MecA
MW, pI	25 kDa, 4.209
Gene length, protein length	654 bp, 218 aa
Immediate neighbours	yjbE, coiA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, proteolysis

The gene

Basic information

• Locus tag: BSU11520

Database entries

• BsubCyc: BSU11520

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mecA family (according to Swiss-Prot)

• Paralogous protein(s): YpbH

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal domain (1 ... 120): recruitment of protein substrates PubMed
  • C-terminal domain (121 ... 218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MecA-ComK PubMed
  • MecA-ComS PubMed
  • ClpC-MecA PubMed
  • MecA-Spo0A PubMed

• Localization:

Database entries

• BsubCyc: BSU11520

• Structure:
  • 3JTP (C-terminal domain) PubMed
  • 3PXG, 3J3U 3J3U (the MecA-ClpC complex) PubMed

• UniProt: P37958

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mecA PubMed

• Expression browser: mecA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)