Difference between revisions of "MecA"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520 mecA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520 mecA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MecA MecA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MecA MecA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 4.209   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 4.209   

Revision as of 16:00, 11 November 2013

  • Description: buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK and ComS to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A∼P by direct interaction

Gene name mecA
Synonyms
Essential no
Product adaptor protein
Function control of ComK degradation, regulation of competence
Gene expression levels in SubtiExpress: mecA
Interactions involving this protein in SubtInteract: MecA
MW, pI 25 kDa, 4.209
Gene length, protein length 654 bp, 218 aa
Immediate neighbours yjbE, coiA
Sequences Protein DNA DNA_with_flanks
Genetic context
MecA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MecA expression.png















Categories containing this gene/protein

genetic competence, proteolysis

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU11520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: mecA family (according to Swiss-Prot)
  • Paralogous protein(s): YpbH

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal domain (1 ... 120): recruitment of protein substrates PubMed
    • C-terminal domain (121 ... 218): facilitates assembly of the ClpC-ClpP oligomer and acts as a degradation tag PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP813 (spc), GP814 (aphA3) both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)

Original Publications