Difference between revisions of "CotW"
| Line 1: | Line 1: | ||
| − | * '''Description:''' spore coat protein (insoluble fraction), formation of the spore crust <br/><br/> | + | * '''Description:''' outer spore coat protein (insoluble fraction), formation of the spore crust <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
| Line 36: | Line 36: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<br/><br/> | <br/><br/> | ||
| Line 47: | Line 43: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[GerE regulon]]}}, | {{SubtiWiki regulon|[[GerE regulon]]}}, | ||
| − | {{SubtiWiki regulon|[[SigK regulon]]}} | + | {{SubtiWiki regulon|[[SigE regulon]]}} |
| + | {{SubtiWiki regulon|[[SigK regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SpoIIID regulon]]}} | ||
=The gene= | =The gene= | ||
| Line 91: | Line 89: | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| + | ** outer spore coat, localization depends on [[CotE]] {{PubMed|22171814}} | ||
=== Database entries === | === Database entries === | ||
| Line 110: | Line 109: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotW_1251273_1251590_-1 cotW] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cotW_1251273_1251590_-1 cotW] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigK]] {{PubMed|15699190,7519271}} | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15383836}} , [[SigK]] {{PubMed|15699190,7519271}} |
| − | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' |
| + | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]], [[GerE]], [[SpoIIID]]) {{PubMed|15383836}} | ||
| + | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
| − | * '''Regulatory mechanism:''' [[GerE]]: transcription activation [ | + | * '''Regulatory mechanism:''' |
| + | ** [[GerE]]: transcription activation {{PubMed|15383836}} | ||
| + | ** [[SpoIIID]]: transcription activation {{PubMed|15383836}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 138: | Line 141: | ||
=References= | =References= | ||
| − | <pubmed>7519271,19304857, 21665972,12107147, </pubmed> | + | <pubmed>7519271,19304857, 21665972,12107147, 15383836 22171814</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:51, 13 August 2013
- Description: outer spore coat protein (insoluble fraction), formation of the spore crust
| Gene name | cotW |
| Synonyms | |
| Essential | no |
| Product | spore coat protein (insoluble fraction) |
| Function | resistance of the spore |
| Gene expression levels in SubtiExpress: cotW | |
| Interactions involving this protein in SubtInteract: CotW | |
| MW, pI | 12 kDa, 9.955 |
| Gene length, protein length | 315 bp, 105 aa |
| Immediate neighbours | cotX, cotV |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
GerE regulon, SigE regulon SigK regulon, SpoIIID regulon
The gene
Basic information
- Locus tag: BSU11770
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: Q08310
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Proteins involved in formation of the outermost layer of Bacillus subtilis spores.
J Bacteriol: 2011, 193(16);4075-80
[PubMed:21665972]
[WorldCat.org]
[DOI]
(I p)
Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857]
[WorldCat.org]
[DOI]
(I p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271]
[WorldCat.org]
[DOI]
(P p)
