Difference between revisions of "GatA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || translation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06680 gatA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06680 gatA]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GatA GatA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GatA GatA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gatC]]'', ''[[gatB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gatC]]'', ''[[gatB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12488&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06680 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06680 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06680 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:gatA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:gatA_context.gif]]

Revision as of 12:18, 13 May 2013

  • Description: production of glutamyl-tRNA(Gln)

Gene name gatA
Synonyms yerM, yedB
Essential yes PubMed
Product glutamyl-tRNA(Gln) amidotransferase subunit A
Function translation
Gene expression levels in SubtiExpress: gatA
Interactions involving this protein in SubtInteract: GatA
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 52 kDa, 5.24
Gene length, protein length 1455 bp, 485 aa
Immediate neighbours gatC, gatB
Sequences Protein DNA Advanced_DNA
Genetic context
GatA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GatA expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06680

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)
  • Protein family: amidase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)