Difference between revisions of "PurK"
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|style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06430 purK] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purE]]'', ''[[purB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purE]]'', ''[[purB]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06430 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06430 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06430 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:purK_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:purK_context.gif]] | ||
Revision as of 12:17, 13 May 2013
- Description: phosphoribosylaminoimidazole carboxylase (ATP-dependent)
| Gene name | purK |
| Synonyms | |
| Essential | no |
| Product | phosphoribosylaminoimidazole carboxylase (ATP-dependent) |
| Function | purine biosynthesis |
| Gene expression levels in SubtiExpress: purK | |
| Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation) | |
| MW, pI | 42 kDa, 5.124 |
| Gene length, protein length | 1137 bp, 379 aa |
| Immediate neighbours | purE, purB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2 (according to Swiss-Prot)
- Protein family: ATP-grasp domain (according to Swiss-Prot)
- Paralogous protein(s): PurT
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 2Z04 (from Aquifex aeolicus, 37% identity, 57% similarity)
- UniProt: P12045
- KEGG entry: [3]
- E.C. number: 4.1.1.21
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093]
[WorldCat.org]
[DOI]
(P p)
M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212]
[WorldCat.org]
[DOI]
(P p)
D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807]
[WorldCat.org]
(P p)
