purT

168

phosphoribosylglycinamide formyltransferase 2

Locus: BSU_02230

Molecular weight: 41.93 kDa

Isoelectric point: 4.72

Protein length: 384 aa

Gene length: 1155 bp

Function: purine biosynthesis

Product: phosphoribosylglycinamide formyltransferase 2

Essential: no

E.C.: null

Synonyms: purT

Genomic Context

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List of homologs in different organisms, belongs to COG0027 (Galperin et al., 2021)

Gene

Coordinates: 243,892 → 245,046

The protein

Catalyzed reaction/ biological activity

• ATP + formate + N¹-(5-phospho-D-ribosyl)glycinamide --> ADP + H⁺ + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)

Protein family

• PurK/PurT family (with PurK, according to UniProt)

Domains

• ATP-grasp domain (aa 111-300) (according to UniProt)

Structure

• 1KJ9 (PDB) (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed
• P39771 (AlphaFold)

Paralogous protein(s)

• PurK

Expression and Regulation

Operons

• Genes: purT

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE02230 (ΔpurT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT,  downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG
• BKK02230 (ΔpurT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT,  downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG

References

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