purS

168

phosphoribosylformylglycinamidine synthase

Locus: BSU_06460

Molecular weight: 9.62 kDa

Isoelectric point: 4.59

Protein length: 84 aa

Gene length: 255 bp

Function: purine biosynthesis

Product: phosphoribosylformylglycinamidine synthase

Essential: no

E.C.: 6.3.5.3

Synonyms: purS, yexA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1828 (Galperin et al., 2021)

Gene

Coordinates: 702,319 702,573

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ADP + H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• UPF0062 family (single member, according to UniProt)

Structure

• 1T4A (PDB)
• P12049 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD

  Description: PubMed

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

  Regulatory mechanism

  • G-box: RNA switch, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A920 (yexA::erm), available at the NBRP B. subtilis, Japan
• BKE06460 (purS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC
• BKK06460 (purS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC

References

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