GatB

• Description: production of glutamyl-tRNA(Gln)
  
  

Gene name	gatB
Synonyms	yerN
Essential	yes PubMed
Product	glutamyl-tRNA(Gln) amidotransferase (sununit B)
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	53 kDa, 4.898
Gene length, protein length	1428 bp, 476 aa
Immediate neighbours	gatA, yerO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06690

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

translation, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate (according to Swiss-Prot)

• Protein family: GatB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GatA-GatB-GatC
  • (GatA-GatB-GatC)-GltX-trnS-Gln (the "transamidosome") PubMed, see a model of the interaction PubMed

• Localization:

Database entries

• Structure:

• UniProt: O30509

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takuhiro Ito, Shigeyuki Yokoyama
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature: 2010, 467(7315);612-6
[PubMed:20882017] [WorldCat.org] [DOI] (I p)

A W Curnow, K w Hong, R Yuan, S i Kim, O Martins, W Winkler, T M Henkin, D Söll
Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation.
Proc Natl Acad Sci U S A: 1997, 94(22);11819-26
[PubMed:9342321] [WorldCat.org] [DOI] (P p)