FlgM

• Description: anti-SigD, regulation of flagellin, motility, and chemotaxis
  
  

Gene name	flgM
Synonyms
Essential	no
Product	anti-SigD
Function	control of SigD activity
MW, pI	9 kDa, 9.918
Gene length, protein length	264 bp, 88 aa
Immediate neighbours	yvyG, yvyF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35430

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: flgM family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: FlgM-SigD PubMed

• Localization:

Database entries

• Structure:

• UniProt: P39809

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • comFA-comFB-comFC-yvyF-flgM-yvyG-flgK-flgL PubMed
  • yvyF-flgM-yvyG-flgK-flgL PubMed

• Sigma factor:
  • comFA: SigA PubMed
  • yvyF: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed
  • ScoC: transcription repression PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Loralyn M Cozy, Daniel B Kearns
Gene position in a long operon governs motility development in Bacillus subtilis.
Mol Microbiol: 2010, 76(2);273-85
[PubMed:20233303] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
A dual mode of regulation of flgM by ScoC in Bacillus subtilis.
Can J Microbiol: 2009, 55(8);983-9
[PubMed:19898538] [WorldCat.org] [DOI] (I p)

M G Bertero, B Gonzales, C Tarricone, F Ceciliani, A Galizzi
Overproduction and characterization of the Bacillus subtilis anti-sigma factor FlgM.
J Biol Chem: 1999, 274(17);12103-7
[PubMed:10207036] [WorldCat.org] [DOI] (P p)

K Fredrick, J D Helmann
FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.
J Bacteriol: 1996, 178(23);7010-3
[PubMed:8955328] [WorldCat.org] [DOI] (P p)

D B Mirel, P Lauer, M J Chamberlin
Identification of flagellar synthesis regulatory and structural genes in a sigma D-dependent operon of Bacillus subtilis.
J Bacteriol: 1994, 176(15);4492-500
[PubMed:8045879] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, D Dubnau
comF, a Bacillus subtilis late competence locus, encodes a protein similar to ATP-dependent RNA/DNA helicases.
Mol Microbiol: 1993, 9(1);119-31
[PubMed:8412657] [WorldCat.org] [DOI] (P p)