Spo0A

• Description: two-component response regulator, initiation of sporulation
  
  

Gene name	spo0A
Synonyms	spo0C, spo0G, spoIIL, sof-1
Essential	no
Product	two-component response regulator
Function	initiation of sporulation
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Ammonium/ glutamate, Central C-metabolism, Sugar catabolism, Stress, tRNA charging
MW, pI	29 kDa, 5.989
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	yqiG, spoIVB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24220

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Genes controlled by Spo0A

• Activation by Spo0A: spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG

• Repression by Spo0A: abrB, kinA, kinC, spo0A, metS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0A-Spo0E, Spo0A-SigA

• Localization:

Database entries

• Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)

• UniProt: P06534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TMB205 (tet), available in the Thorsten Mascher lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Tony Wilkinson, York University, U.K. homepage
• Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage
• Charles Moran, Emory University, NC, USA homepage