Difference between revisions of "AcoB"

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=Expression and regulation=
 
=Expression and regulation=
 
 
* '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
 
* '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
  
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
 
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
  
* '''Regulation:''' repressed by glucose ([[CcpA]]) induced by acetoin ([[AcoR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
+
* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]]) {{PubMed|10666464}}
 +
** induced by acetoin ([[AcoR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
 +
** induced under anaerobic conditions ([[Fnr]]) {{PubMed|16428414}}
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression[[AcoR]]: transcription activation (interaction with [[SigL]]-containing RNA polymerase) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
+
* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression {{PubMed|10666464}}
 +
** [[AcoR]]: transcription activation (interaction with [[SigL]]-containing RNA polymerase) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109  PubMed]
 +
** [[Fnr]]: indirect positive regulation via [[NarG]]-[[NarH]]-[[NarJ]]-[[NarI]] {{PubMed|16428414}}
  
* '''Additional information:''' the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
+
* '''Additional information:'''the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
  
 
=Biological materials =
 
=Biological materials =
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<pubmed>11274109,10368162,,12884008,16479537,11274109 </pubmed>
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<pubmed>11274109,10368162,10666464,12884008,16479537,11274109 16428414</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:53, 14 May 2010

  • Description: acetoin dehydrogenase E1 component (TPP-dependent beta subunit)

Gene name acoB
Synonyms yfjJ
Essential no
Product acetoin dehydrogenase E1 component
(TPP-dependent beta subunit)
Function acetoin utilization
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 36 kDa, 4.396
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours acoA, acoC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AcoB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU08070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: Membrane-proximal (Spotty) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References