Difference between revisions of "HrcA"

From SubtiWiki
Jump to: navigation, search
Line 80: Line 80:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P25499 P25499]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P25499 P25499]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25490]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25490]

Revision as of 13:41, 20 July 2009

  • Description: transcriptional repressor of class I heat-shock genes

Gene name hrcA
Synonyms yqxE, yqeS
Essential no
Product transcriptional repressor
Function regulation of chaperone gene expression
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 38 kDa, 6.079
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours grpE, hemN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HrcA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU25490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription repressor of the hrcA-grpE-dnaK-dnaJ and groES-groEL operons (at low/ normal temperatures)
  • Protein family: hrcA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): GroEL acts as co-repressor
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: repressed at normal temperature by HrcA
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

Your additional remarks

References

Thomas Wiegert, Wolfgang Schumann
Analysis of a DNA-binding motif of the Bacillus subtilis HrcA repressor protein.
FEMS Microbiol Lett: 2003, 223(1);101-6
[PubMed:12799007] [WorldCat.org] [DOI] (P p)

Silke Reischl, Thomas Wiegert, Wolfgang Schumann
Isolation and analysis of mutant alleles of the Bacillus subtilis HrcA repressor with reduced dependency on GroE function.
J Biol Chem: 2002, 277(36);32659-67
[PubMed:12082092] [WorldCat.org] [DOI] (P p)

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

A Mogk, G Homuth, C Scholz, L Kim, F X Schmid, W Schumann
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.
EMBO J: 1997, 16(15);4579-90
[PubMed:9303302] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

A Schulz, W Schumann
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.
J Bacteriol: 1996, 178(4);1088-93
[PubMed:8576042] [WorldCat.org] [DOI] (P p)

A Schulz, B Tzschaschel, W Schumann
Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis.
Mol Microbiol: 1995, 15(3);421-9
[PubMed:7540247] [WorldCat.org] [DOI] (P p)

U Zuber, W Schumann
CIRCE, a novel heat shock element involved in regulation of heat shock operon dnaK of Bacillus subtilis.
J Bacteriol: 1994, 176(5);1359-63
[PubMed:8113175] [WorldCat.org] [DOI] (P p)

M Wetzstein, U Völker, J Dedio, S Löbau, U Zuber, M Schiesswohl, C Herget, M Hecker, W Schumann
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
J Bacteriol: 1992, 174(10);3300-10
[PubMed:1339421] [WorldCat.org] [DOI] (P p)