LevE

• Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component
  
  

Gene name	levE
Synonyms	sacL
Essential	no
Product	trigger enzyme: fructose-specific phosphotransferase system, EIIB component fructose-specific enzyme IIB (EC 2.7.1.69)
Function	fructose uptake and phosphorylation, control of LevR activity
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	17 kDa, 9.444
Gene length, protein length	486 bp, 162 aa
Immediate neighbours	levF, levD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, mannose permease (Man) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LevE-LevR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1BLE

• Swiss prot entry: P26380

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: levD-levE-levF-levG-sacC

• Sigma factor: SigL

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose

• Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: