Difference between revisions of "SacC"
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=== Basic information === | === Basic information === | ||
| − | * ''' | + | * '''Locus tag:''' |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Revision as of 15:39, 2 June 2009
- Description: levanase
| Gene name | sacC |
| Synonyms | sacL |
| Essential | no |
| Product | levanase |
| Function | degradation of levan to fructose |
| MW, pI | 75 kDa, 6.789 |
| Gene length, protein length | 2031 bp, 677 aa |
| Immediate neighbours | yraA, levG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P05656
- KEGG entry: BSU27030
- E.C. number: 3.2.1.80
Additional information
Expression and regulation
- Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose
- Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Martin, I., Débarbouillé, M., Ferrari, E., Klier, A., and Rapoport, G. (1987) Characterization of the levanase gene of Bacillus subtilis which shows extensive homology to yeast invertase. Mol Gen Genet 208: 177-184. PubMed
- Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
